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Product Name
CLN6 antibody
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Description
CLN6 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in SH-SY5Y cells, HEK-293 cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human colon tissue, human small intestine tissue. Observed molecular weight by Western-blot: 27-37 kDa
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Tested applications
ELISA, IP, IHC, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
CLN6 antibody; FLJ20561 antibody; HsT18960 antibody; nclf antibody; Protein CLN6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLN6 recombinant protein (Accession Number: NM_017882). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:109397(CLN6 Antibody) at dilution of 1:1000
IP Result of anti-CLN6 (IP:Catalog No:109397, 4ug; Detection:Catalog No:109397 1:300) with HEK-293 cells lysate 2560ug.
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109397(CLN6 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109397(CLN6 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:109397(CLN6 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CLN6 represents ceroid-lipofuscinosis, neuronal 6, late infantile, variant
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