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Product Name
CLIP1 antibody
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Description
CLIP1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, MCF7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 160-170 kDa, 135 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CLIP antibody; CLIP 170 antibody; CLIP1 antibody; CLIP170 antibody; CYLN1 antibody; Cytoplasmic linker protein 1 antibody; Restin antibody; RSN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLIP1 recombinant protein (Accession Number: NM_001247997). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109392(CLIP1 antibody) at dilution of 1:2000
IP Result of anti-CLIP1 (IP:Catalog No:109392, 4ug; Detection:Catalog No:109392 1:2000) with HeLa cells lysate 2400ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109392(CLIP1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CLIP1 represents CAP-GLY domain containing linker protein 1
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