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Product Name
Claudin 9-specific antibody
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Description
Claudin 9-specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
claudin 9 antibody; CLDN9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020982). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109343(CLDN9-specific antibody) at dilution of 1:100
IP Result of anti-CLDN9-specific (IP:Catalog No:109343, 4ug; Detection:Catalog No:109343 1:300) with HeLa cells lysate 880ug.
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Background
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.
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References
- Kratzer I, Vasiljevic A, Rey C. Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier. Histochemistry and cell biology. 138(6):861-79. 2012.
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