-
Product Name
Claspin antibody
- Documents
-
Description
Claspin Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IF detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 240 kDa
-
Tested applications
ELISA, WB, IF, IP
-
Species reactivity
Human; other species not tested.
-
Alternative names
CLASPIN antibody; CLSPN antibody; hClaspin antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Claspin recombinant protein (Accession Number: NM_022111). Purification method: Antigen Affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:500-1:5000
IF: 1:20-1:200
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109335(CLSPN Antibody) at dilution of 1:300
IP Result of anti-CLSPN (IP:Catalog No:109335, 5ug; Detection:Catalog No:109335 1:1000) with HEK-293 cells lysate 2400ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:109335(CLSPN Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
-
Background
CLSPN, also named as Claspin, is a 1339 amino acid protein, which belongs to the claspin family. CLSPN localizes in the nucleus and is highly expressed at S/G2 phases of the cell cycle. CLSPN is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. CLSPN plays a potentially critical role in replication checkpoint control in cells and mediating phosphorylation and activation of checkpoint protein kinases. The calculated molecular weight of CLSPN is 151 kDa, but the modified protein is about 220-240 kDa.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"