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Product Name
CLASP1 antibody
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Description
CLASP1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in rat brain tissue, human brain tissue. Positive IF detected in Hela cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 162kd
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Tested applications
ELISA, IF, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CLASP1 antibody; CLIP assOCiating protein 1 antibody; DKFZp686D1968 antibody; DKFZp686H2039 antibody; FLJ33821 antibody; FLJ41222 antibody; hOrbit1 antibody; KIAA0622 antibody; MAST1 antibody; Multiple asters homolog 1 antibody; Protein Orbit homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLASP1 recombinant protein (Accession Number: XM_047443786). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109333(CLASP1 Antibody) at dilution of 1:600
IP Result of anti-CLASP1 (IP:Catalog No:109333, 4ug; Detection:Catalog No:109333 1:500) with HeLa cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human testis using Catalog No:109333(CLASP1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using CLASP1 antibody Catalog No:109333 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CLASP1 is a member of CLASP family. Members of the CLASP family of proteins are involved in multiple MT-dependent processes, including Golgi complex organization and trafficking, kinetochore fiber dynamics and flux, mitotic spindle assembly, and the stabilization of MT plus ends near the cortex. CLASP proteins regulate MT dynamics by promoting MT rescue and suppressing MT catastrophe. Recent work showed that CLASP1 activity is required for proper positioning of the mitotic spindle in both the C. elegans embryo and mammalian cells. Recent work has also identified roles CLASP1 in the regulation of spindle positioning.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"