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Product Name
CIAO1 antibody
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Description
CIAO1 Rabbit Polyclonal antibody. Positive IHC detected in human small intestine tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, MCF-7 cells, PC-3 cells. Observed molecular weight by Western-blot: 48-55 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
CIA1 antibody; CIAO1 antibody; WDR39 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CIAO1 recombinant protein (Accession Number: NM_004804). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109307(CIAO1 Antibody) at dilution of 1:4000
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109307(CIAO1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:109307(CIAO1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:109307(CIAO1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109307(CIAO1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CIAO1, also named as CIA1 or WDR39, is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. It may also play a role in chromosome segregation. The predicted MW of this protein is 38 kDa. 10295-1-AP detected 48-55 kDa band because of phosphorylation. And 10295-1-AP do well in IHC and IF detection.
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