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Product Name
Chymotrypsin like protease antibody
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Description
Chymotrypsin like protease Rabbit Polyclonal antibody. Positive WB detected in mouse pancreas tissue, BxPC-3 cells. Positive IHC detected in human pancreas tissue. Positive IF detected in BxPC-3 cells. Observed molecular weight by Western-blot: 25-28kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
chymotrypsin like antibody; Chymotrypsin like protease CTRL 1 antibody; CTRL antibody; CTRL1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Chymotrypsin like protease recombinant protein (Accession Number: NM_001907). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:109306(CTRL antibody) at dilution of 1:3000
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:109306(CTRL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:109306(CTRL antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of BxPC-3 cells using Catalog No:109306(CTRL Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.
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