Product Name

Chromogranin B antibody

Documents

Description

Chromogranin B Rabbit Polyclonal antibody. Positive IF detected in PC-12 cells. Positive IHC detected in human pancreas tissue. Positive WB detected in PC-12 cells, mouse brain tissue. Observed molecular weight by Western-blot: 100 kDa

Tested applications

ELISA, WB, IHC, IF

Species reactivity

Human, Mouse; other species not tested.

Alternative names

CgB antibody; CHGB antibody; Chromogranin B antibody; SCG1 antibody; Secretogranin 1 antibody; Secretogranin I antibody; SgI antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Chromogranin B recombinant protein (Accession Number: NM_001819). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:1000

IHC: 1:20-1:200

IF: 1:50-1:500

Validations

PC-12 cells were subjected to SDS PAGE followed by western blot with Catalog No:109289(CHGB Antibody) at dilution of 1:300

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:109289(CHGB Antibody) at dilution of 1:200 (under 10x lens)

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:109289(CHGB Antibody) at dilution of 1:200 (under 40x lens)

Immunofluorescent analysis of (-20oc Ethanol) fixed PC-12 cells using Catalog No:109289(CHGB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

CHGB represents chromogranin B (secretogranin 1)

References

Collins MA, An J, Hood BL, Conrads TP, Bowser RP. Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis. Journal of proteome research. 14(11):4486-501. 2015.

Chromogranin B antibody

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