-
Product Name
CHRNA7 antibody
- Documents
-
Description
CHRNA7 Rabbit Polyclonal antibody. Positive WB detected in rat brain tissue, MCF-7 cells, mouse skeletal muscle tissue. Positive FC detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 56 kDa
-
Tested applications
ELISA, WB, FC
-
Species reactivity
Human, Rat; other species not tested.
-
Alternative names
CHRNA7 antibody; CHRNA7 2 antibody; NACHRA7 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CHRNA7 recombinant protein (Accession Number: XM_017021884). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
-
Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109282(CHRNA7 Antibody) at dilution of 1:600
1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNA7 antibody (Catalog No:109282, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
-
Background
Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"