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Product Name
CHMP2B antibody
- Documents
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Description
CHMP2B Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, human brain tissue, human heart tissue, human ileum tissue, human kidney tissue, human placenta tissue, mouse brain tissue, NIH/3T3 cells. Positive IF detected in HepG2 cells, PFA fixed cells. Positive IHC detected in human liver tissue, human brain tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CHMP2.5 antibody; CHMP2B antibody; chromatin modifying protein 2B antibody; DKFZp564O123 antibody; DMT1 antibody; hVps2 2 antibody; VPS2 2 antibody; VPS2B antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHMP2B recombinant protein (Accession Number: NM_014043). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:109234(CHMP2B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:109234(CHMP2B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:109234(CHMP2B antibody) at dilution of 1:50 (under 40x lens)
IF result (endosomal stain) of anti-CHMP2B (Catalog No:109234) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman.
Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody Catalog No:109234 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
CHMP2B, chromatin-modifying protein 2b, also named as CHMP2.5, VPS2B and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or been replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, maybe caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.
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References
- Cheng CM, Wang D, Cao X, Luo QQ, Lu YP, Zhu L. Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells. Journal of cellular biochemistry. 116(9):1919-31. 2015.
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