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Product Name
CHMP2A antibody
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Description
CHMP2A Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CHMP2 antibody; CHMP2A antibody; chromatin modifying protein 2A antibody; hVps2 1 antibody; VPS2 antibody; Vps2 1 antibody; VPS2A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHMP2A recombinant protein (Accession Number: NM_014453). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109233(CHMP2A antibody) at dilution of 1:300
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Background
CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).
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References
- Olmos Y, Hodgson L, Mantell J, Verkade P, Carlton JG. ESCRT-III controls nuclear envelope reformation. Nature. 522(7555):236-9. 2015.
- Vietri M, Schink KO, Campsteijn C. Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing. Nature. 522(7555):231-5. 2015.
- Kuang Z, Seo EJ, Leis J. Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15. Journal of virology. 85(14):7153-61. 2011.
- Lee S, Chang J, Renvoisé B, Tipirneni A, Yang S, Blackstone C. MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis. Molecular biology of the cell. 23(22):4347-61. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"