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Product Name
Chk1 antibody
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Description
Chk1 Mouse Monoclonal antibody. Positive IHC detected in human small intestine tissue, human testis tissue. Positive WB detected in HeLa cells, human testis tissue. Observed molecular weight by Western-blot: 40 kDa,50 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CHEK1 antibody; Chk1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Chk1 recombinant protein (Accession Number: NM_001114122). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107154(CHK1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:107154(CHK1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:107154(CHK1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.
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