CHDH antibody

Cat.#: 109222

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Product Information

  • Product Name
    CHDH antibody
  • Documents
  • Description
    CHDH Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HepG2 cells, mouse kidney tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 60 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CDH antibody; CHD antibody; CHDH antibody; Choline dehydrogenase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CHDH recombinant protein (Accession Number: NM_018397). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:20-1:200

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109222(CHDH antibody) at dilution of 1:800

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109222(CHDH antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human liver using Catalog No:109222(CHDH antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:109222(CHDH antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:109222(CHDH antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:109222(CHDH antibody) at dilution of 1:100 (under 40x lens)

  • Background
    CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.
  • References
    • Johnson AR, Lao S, Wang T, Galanko JA, Zeisel SH. Choline dehydrogenase polymorphism rs12676 is a functional variation and is associated with changes in human sperm cell function. PloS one. 7(4):e36047. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"