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Product Name
CHDH antibody
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Description
CHDH Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HepG2 cells, mouse kidney tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDH antibody; CHD antibody; CHDH antibody; Choline dehydrogenase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHDH recombinant protein (Accession Number: NM_018397). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109222(CHDH antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human liver using Catalog No:109222(CHDH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:109222(CHDH antibody) at dilution of 1:100 (under 40x lens)
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Background
CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.
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References
- Johnson AR, Lao S, Wang T, Galanko JA, Zeisel SH. Choline dehydrogenase polymorphism rs12676 is a functional variation and is associated with changes in human sperm cell function. PloS one. 7(4):e36047. 2012.
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