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Product Name
CHD3 antibody
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Description
CHD3 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IHC detected in human kidney tissue, human colon tissue, human ovary tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 240 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ATP dependent helicase CHD3 antibody; CHD 3 antibody; CHD3 antibody; hZFH antibody; Mi 2a antibody; Mi2 ALPHA antibody; ZFH antibody; Zinc finger helicase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005852). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109219(CHD3 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109219(CHD3 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:109219(CHD3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:109219(CHD3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.
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