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Product Name
CHCHD4 antibody
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Description
CHCHD4 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive IF detected in Hela cells. Positive IP detected in mouse brain tissue. Positive WB detected in rat brain tissue, human heart tissue, L02 cells, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse spleen tissue. Observed molecular weight by Western-blot: 22-25 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CHCHD4 antibody; FLJ31709 antibody; MIA40 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHCHD4 recombinant protein (Accession Number: NM_001098502). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109214(CHCHD4 Antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109214(CHCHD4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109214(CHCHD4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CHCHD4 antibody Catalog No:109214 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CHCHD4 (IP:Catalog No:109214, 4ug; Detection:Catalog No:109214 1:500) with mouse brain tissue lysate 4000ug.
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Background
CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).
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References
- Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.
- Meyer K, Buettner S, Ghezzi D, Zeviani M, Bano D, Nicotera P. Loss of apoptosis-inducing factor critically affects MIA40 function. Cell death & disease. 6:e1814. 2015.
- Balogh T, Lőrincz T, Stiller I, Mandl J, Bánhegyi G, Szarka A. The Level of ALR is Regulated by the Quantity of Mitochondrial DNA. Pathology oncology research : POR. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"