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Product Name
CHAT antibody
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Description
CHAT Rabbit Polyclonal antibody. Positive WB detected in rat brain tissue, human placenta tissue, Jurkat cells, mouse placenta tissue. Positive IHC detected in rat brain tissue, human brain tissue, human stomach tissue, mouse brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 47-50, 66-70 kDa
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Tested applications
ELISA, IF, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CHAT antibody; CHOACTase antibody; Choline acetylase antibody; choline acetyltransferase antibody; Choline O acetyltransferase antibody; CMS1A antibody; CMS1A2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020549). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded rat brain using Catalog No:109209(CHAT antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded rat brain using Catalog No:109209(CHAT antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CHAT antibody Catalog No:109209 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109209(CHAT Antibody) at dilution of 1:600
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Background
CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively. This antibody mainly recognizes the 45-50 kDa pCHAT in multiple lysates.
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References
- Hou XQ, Zhang L, Yang C. Alleviating effects of Bushen-Yizhi formula on ibotenic acid-induced cholinergic impairments in rat. Rejuvenation research. 18(2):111-27. 2015.
- Olivas A, Gardner RT, Wang L, Ripplinger CM, Woodward WR, Habecker BA. Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130. The Journal of neuroscience : the official journal of the Society for Neuroscience. 36(2):479-88. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"