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Product Name
CFLAR/FLIP antibody
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Description
CFLAR/FLIP Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells. Positive IP detected in Jurkat cells. Positive IHC detected in human skeletal muscle tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 30-35 kDa,50-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
c FLIP antibody; c FLIPL antibody; c FLIPR antibody; c FLIPS antibody; CASH antibody; CASP8AP1 antibody; Caspase eight related protein antibody; Caspase homolog antibody; Casper antibody; CFLAR antibody; CLARP antibody; FLAME antibody; FLAME 1 antibody; FLAME1 antibody; FLIP antibody; I FLICE antibody; Inhibitor of FLICE antibody; MRIT antibody; Usurpin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CFLAR/FLIP recombinant protein (Accession Number: XM_017005191). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109199(CFLAR antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:109199(CFLAR Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:109199(CFLAR Antibody) at dilution of 1:50
IP Result of anti-CFLAR (IP:Catalog No:109199, 4ug; Detection:Catalog No:109199 1:500) with Jurkat cells lysate 4000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:109199(CFLAR Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.
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References
- Zhang X, Jiang SJ, Shang B, Jiang HJ. Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line. Thoracic cancer. 6(2):202-8. 2015.
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