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Product Name
CEP164 antibody
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Description
CEP164 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IP detected in RPE1 cells. Positive IF detected in MDCK cells, HeLa cells. Positive IHC detected in human colon tissue, human cervical cancer tissue. Observed molecular weight by Western-blot: 164 kDa
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Tested applications
ELISA, IP, IF, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
centrosomal protein 164kDa antibody; Centrosomal protein of 164 kDa antibody; CEP164 antibody; FLJ54767 antibody; KIAA1052 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CEP164 recombinant protein (Accession Number: BC000602). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:500-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
WB results of CEP164 (Catalog No:109267, 1:500) with RPE1 cells by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.
IF results of CEP164 (Catalog No:109267, 1:1000) with HeLa cells (1.5% formaldehyde, 10 min RT) by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109267(CEP164 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:109267(CEP164 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MDCK cells using Catalog No:109267(CEP164 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CEP164, also named as KIAA1052 or NPHP15, is a 1460 amino acid protein, which contains 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.
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References
- Stephen LA, Tawamie H, Davis GM. TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). eLife. 4:. 2015.
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