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Product Name
CDK9 antibody
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Description
CDK9 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in A431 cells, HEK-293 cells, human placenta tissue, Jurkat cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C 2k antibody; CDC2L4 antibody; CDK9 antibody; Cell division protein kinase 9 antibody; CTK1 antibody; cyclin dependent kinase 9 antibody; PITALRE antibody; TAK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDK9 recombinant protein (Accession Number: NM_001261). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109168(CDK9 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109168(CDK9 antibody) at dilution of 1:50 (under 40x lens)
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Background
CDK9(Cyclin-dependent kinase 9) is involved in the regulation of transcription.It has two isforms.CDK9-55 had an apparent molecular mass of 55 kD. The ratio of CDK9-55 to the shorter CDK9 isoform, CDK9-42, differed between HeLa cell nuclear extracts and mouse fibroblasts, but CDK9-42 was the predominant isoform in both(PMID:12706900).CDK9 is located primarily in the nucleus.
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