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Product Name
CDK2 antibody
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Description
CDK2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in K-562 cells, A431 cells, HEK-293 cells, HeLa cells, HL-60 cells, Jurkat cells, MCF7 cells, NIH/3T3 cells, rat kidney tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IF, IP, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CDK2 antibody; Cell division protein kinase 2 antibody; cyclin dependent kinase 2 antibody; p33 protein kinase antibody; p33(CDK2) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDK2 recombinant protein (Accession Number: NM_001798). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109158(CDK2 antibody) at dilution of 1:600
IP Result of anti-CDK2 (IP:Catalog No:109158, 3ug; Detection:Catalog No:109158 1:1500) with HeLa cells lysate 4650ug.
Immunofluorescent analysis of HepG2 cells, using CDK2 antibody Catalog No:109158 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with 0.2ug CDK2 antibody (Catalog No:109158, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
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References
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- Xiao W, Jiang Y, Men Q. Tetrandrine induces G1/S cell cycle arrest through the ROS/Akt pathway in EOMA cells and inhibits angiogenesis in vivo. International journal of oncology. 46(1):360-8. 2015.
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