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Product Name
CDCA3 antibody
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Description
CDCA3 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IHC detected in human cervical cancer tissue.
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CDCA3 antibody; Gene rich cluster protein C8 antibody; GRCC8 antibody; TOME 1 antibody; TOME1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDCA3 recombinant protein (Accession Number: NM_031299). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109114(CDCA3 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109114(CDCA3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109114(CDCA3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
CDCA3, also named as C8, GRCC8 and TOME1, is an F-box-like protein which required for entry into mitosis. It acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"