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Product Name
CDC27; APC3 antibody
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Description
CDC27; APC3 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human prostate cancer tissue. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, Jurkat cells. Observed molecular weight by Western-blot: 92kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ANAPC3 antibody; APC3 antibody; CDC27 antibody; CDC27 homolog antibody; CDC27Hs antibody; D0S1430E antibody; D17S978E antibody; H NUC antibody; HNUC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDC27; APC3 recombinant protein (Accession Number: NM_001114091). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109100(CDC27; APC3 antibody) at dilution of 1:600
IP Result of anti-CDC27; APC3 (IP:Catalog No:109100, 3ug; Detection:Catalog No:109100 1:600) with K-562 cells lysate 11000ug.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109100(CDC27; APC3 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody Catalog No:109100 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.
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