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Product Name
CD9 antibody
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Description
CD9 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in human placenta tissue, L02 cells. Observed molecular weight by Western-blot: 23-27 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
5H9 antibody; 5H9 antigen antibody; BA2 antibody; BTCC 1 antibody; CD9 antibody; CD9 antigen antibody; CD9 molecule antibody; DRAP 27 antibody; GIG2 antibody; LeukOCyte antigen MIC3 antibody; MIC3 antibody; Motility related protein antibody; MRP 1 antibody; P24 antibody; Tetraspanin 29 antibody; Tspan 29 antibody; TSPAN29 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD9 recombinant protein (Accession Number: NM_001769). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:109147(CD9 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:109147(CD9 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:109147(CD9 Antibody) at dilution of 1:50
1X10^6 HeLa cells were stained with .2ug CD9 antibody (Catalog No:109147, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
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