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CD86 antibody

Cat.#: 109143

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD86 antibody
  • Documents
  • Description
    CD86 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, Raji cells. Positive FC detected in Raji cells. Observed molecular weight by Western-blot: 70 kDa
  • Tested applications
    ELISA, WB, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    Activation B7 2 antigen antibody; B7 2 antibody; B70 antibody; BU63 antibody; CD28LG2 antibody; CD86 antibody; CD86 molecule antibody; CTLA 4 counter receptor B7.2 antibody; FUN 1 antibody; LAB72 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD86 recombinant protein (Accession Number: NM_175862). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109143(CD86 antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109143(CD86 antibody) at dilution of 1:500

    1X10^6 Raji cells were stained with 0.2ug CD86 antibody (Catalog No:109143, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Raji cells were stained with 0.2ug CD86 antibody (Catalog No:109143, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"