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Product Name
CD47 antibody
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Description
CD47 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 47-55 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CD47 antibody; CD47 molecule antibody; IAP antibody; LeukOCyte surface antigen CD47 antibody; MER6 antibody; OA3 antibody; Protein MER6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD47 recombinant protein (Accession Number: NM_198793). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:50-1:500
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109127(CD47 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:109127(CD47 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:109127(CD47 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
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