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CD43 antibody

Cat.#: 109123

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD43 antibody
  • Documents
  • Description
    CD43 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in K-562 cells, Jurkat cells. Observed molecular weight by Western-blot: 60-66 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CD43 antibody; Galactoglycoprotein antibody; GALGP antibody; GPL115 antibody; LeukOCyte sialoglycoprotein antibody; Leukosialin antibody; LSN antibody; sialophorin antibody; SPN antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD43 recombinant protein (Accession Number: NM_001030288). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109123(SPN antibody) at dilution of 1:500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109123(SPN antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109123(SPN antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109123(SPN antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109123(SPN antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109123(SPN antibody) at dilution of 1:50 (under 40x lens)

  • Background
    CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"