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Product Name
CD23 antibody
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Description
CD23 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive FC detected in Raji cells. Positive WB detected in Raji cells. Observed molecular weight by Western-blot: 38-42 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
BLAST 2 antibody; CD23 antibody; CD23A antibody; CLEC4J antibody; Fc epsilon RII antibody; FCE2 antibody; FCER2 antibody; IGEBF antibody; LymphOCyte IgE receptor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD23 recombinant protein (Accession Number: NM_001220500). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109010(FCER2,CD23 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109010(FCER2,CD23 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109010(FCER2,CD23 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 Raji cells were stained with 0.2ug FCER2,CD23 antibody (Catalog No:109010, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"