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Product Name
CD23 antibody
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Description
CD23 Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive WB detected in Hela cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
BLAST 2 antibody; CD23 antibody; CD23A antibody; CLEC4J antibody; Fc epsilon RII antibody; FCE2 antibody; FCER2 antibody; IGEBF antibody; LymphOCyte IgE receptor antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of CD23 recombinant protein (Accession Number: BC064417). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107126(CD23,FCER2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107126(CD23,FCER2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107126(CD23,FCER2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"