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Product Name
CD18 antibody
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Description
CD18 Rabbit Polyclonal antibody. Positive WB detected in U-937 cells. Positive FC detected in RAW 264.7 cells. Positive IHC detected in human tonsillitis tissue. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, FC, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CD18 antibody; Integrin beta 2 antibody; ITGB2 antibody; LAD antibody; LCAMB antibody; LFA 1 antibody; MAC 1 antibody; MF17 antibody; MFI7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD18 recombinant protein (Accession Number: NM_000211). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:50-1:500
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Validations
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:109062(CD18 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109062(CD18 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109062(CD18 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
1X10^6 RAW 264.7 cells were stained with 0.2ug CD18 antibody (Catalog No:109062, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ITGB2 represents integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
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