-
Product Name
CD154 antibody
- Documents
-
Description
CD154 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human spleen tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human testis tissue. Positive IP detected in Jurkat cells. Positive WB detected in mouse small intestine tissue, HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 29-32 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
CD154 antibody; CD40 L antibody; CD40 ligand antibody; CD40L antibody; CD40LG antibody; gp39 antibody; hCD40L antibody; HIGM1 antibody; IGM antibody; IMD3 antibody; T BAM antibody; T cell antigen Gp39 antibody; TNF related activation protein antibody; TNFSF5 antibody; TRAP antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CD154 recombinant protein (Accession Number: NM_000074). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:109057(CD40LG antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109057(CD40L; CD154 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109057(CD40LG antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109057(CD40LG antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CD40L; CD154 (IP:Catalog No:109057, 4ug; Detection:Catalog No:109057 1:1000) with Jurkat cells lysate 3200ug.
-
Background
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.
-
References
- Bian F, Yang X, Zhou F. C-reactive protein promotes atherosclerosis by increasing LDL transcytosis across endothelial cells. British journal of pharmacology. 171(10):2671-84. 2014.
- Zhang Y, Yang X, Bian F. TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ. Journal of molecular and cellular cardiology. 72:85-94. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"