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Product Name
CD11B/Integrin alpha M antibody
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Description
CD11B/Integrin alpha M Rabbit Polyclonal antibody. Positive FC detected in HL-60 cells. Positive IHC detected in human spleen tissue, human tonsillitis tissue. Positive WB detected in HL-60 cells, Jurkat cells. Observed molecular weight by Western-blot: 110-170 kDa
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Tested applications
ELISA, IHC, WB, FC
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Species reactivity
Human; other species not tested.
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Alternative names
CD11B antibody; CR 3 alpha chain antibody; CR3A antibody; Integrin alpha M antibody; ITGAM antibody; MAC 1 antibody; MAC1A antibody; MO1A antibody; Neutrophil adherence receptor antibody; SLEB6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000632). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109041(CD11B/Integrin alpha M Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109041(CD11B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109041(CD11B antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HL-60 cells were stained with 0.2ug CD11B/Integrin alpha M antibody (Catalog No:109041, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ITGAM, also named as CD11B and CR3A, belongs to the integrin alpha chain family. ITGAM is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. ITGAM is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The antibody is specific to ITGAM.
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