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Product Name
CCT5 antibody
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Description
CCT5 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, COLO 320 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
CCT epsilon antibody; CCT5 antibody; CCTE antibody; KIAA0098 antibody; TCP 1 epsilon antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CCT5 recombinant protein (Accession Number: NM_012073). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109084(CCT5 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109084(CCT5 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CCT5 antibody Catalog No:109084 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CCT5 (IP:Catalog No:109084, 4ug; Detection:Catalog No:109084 1:1000) with HeLa cells lysate 1200ug.
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Background
CCT5 represents chaperonin containing TCP1, subunit 5 (epsilon)
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References
- Seo S, Baye LM, Schulz NP. BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. Proceedings of the National Academy of Sciences of the United States of America. 107(4):1488-93. 2010.
- Yamabe K, Maeda H, Kokeguchi S. Antigenic group II chaperonin in Methanobrevibacter oralis may cross-react with human chaperonin CCT. Molecular oral microbiology. 25(2):112-22. 2010.
- Tarkar A, Loges NT, Slagle CE. DYX1C1 is required for axonemal dynein assembly and ciliary motility. Nature genetics. 45(9):995-1003. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"