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Product Name
CCR2-specific antibody
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Description
CCR2-specific Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A431 cells. Positive FC detected in Jurkat cells. Positive IHC detected in human kidney tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
C C chemokine receptor type 2 antibody; C C CKR 2 antibody; CC CKR 2 antibody; CCR 2 antibody; CCR2 antibody; CCR2a antibody; CCR2b antibody; CD192 antibody; CKR2 antibody; CKR2A antibody; CKR2B antibody; CMKBR2 antibody; FLJ78302 antibody; MCP 1 R antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000647). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109075(CCR2a-specific antibody) at dilution of 1:200
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:109075(CCR2a-specific Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:109075(CCR2a-specific Antibody) at dilution of 1:50
1X10^6 Jurkat cells were stained with 0.2ug CCR2a-specific antibody (Catalog No:109075, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specicifc to CCR2a.
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