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Product Name
CCNY antibody
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Description
CCNY Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in K-562 cells, A549 cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 33-39 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C10orf9 antibody; CBCP1 antibody; CCNY antibody; CFP1 antibody; Cyc Y antibody; Cyclin box protein 1 antibody; Cyclin fold protein 1 antibody; cyclin X antibody; cyclin Y antibody; FLJ95513 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CCNY recombinant protein (Accession Number: XM_011519358). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109071(CCNY antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells, using CCNY antibody Catalog No:109071 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CCNY (IP:Catalog No:109071, 3ug; Detection:Catalog No:109071 1:600) with K-562 cells lysate 2400ug.
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Background
CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.
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References
- Cho E, Kim DH, Hur YN. Cyclin Y inhibits plasticity-induced AMPA receptor exocytosis and LTP. Scientific reports. 5:12624. 2015.
- Shehata SN, Hunter RW, Ohta E. Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase. Cellular signalling. 24(11):2085-94. 2012.
- Shehata SN, Deak M, Morrice NA. Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16. The Biochemical journal. 469(3):409-20. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"