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Product Name
CBX3 antibody
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Description
CBX3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, A375 cells, mouse spleen tissue. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CBX3 antibody; Chromobox protein homolog 3 antibody; HECH antibody; HP1 GAMMA antibody; HP1Hs gamma antibody; Modifier 2 protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CBX3 recombinant protein (Accession Number: NM_007276). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108945(CBX3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108945(CBX3 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CBX3 antibody Catalog No:108945 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CBX3 (IP:Catalog No:108945, 4ug; Detection:Catalog No:108945 1:500) with mouse spleen tissue lysate 4000ug.
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Background
The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins
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References
- Jiang WQ, Zhong ZH, Nguyen A. Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins. The Journal of cell biology. 185(5):797-810. 2009.
- Han SS, Kim WJ, Hong Y. RNA sequencing identifies novel markers of non-small cell lung cancer. Lung cancer (Amsterdam, Netherlands). 84(3):229-35. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"