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Product Name
CBLL1 antibody
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Description
CBLL1 Rabbit Polyclonal antibody. Positive WB detected in DU 145 cells, HEK-293 cells, HeLa cells, MCF7 cells, L02 cells. Positive IF detected in HepG2. Positive IHC detected in human breast cancer. Observed molecular weight by Western-blot: 56kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
CBLL1 antibody; RNF188 antibody; c Cbl like protein 1 antibody; RING finger protein 188 antibody; FLJ23109 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CBLL1 recombinant protein (Accession Number: NM_024814). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
DU 145 cells were subjected to SDS PAGE followed by western blot with Catalog No:108901(CBLL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108901(CBLL1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108901(CBLL1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CBLL1 antibody Catalog No:108901 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CBLL1 represents Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
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References
- Sako-Kubota K, Tanaka N, Nagae S, Meng W, Takeichi M. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Molecular biology of the cell. 25(24):3851-60. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"