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Caspase 3, p17-specific antibody

Cat.#: 108875

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Product Information

  • Product Name
    Caspase 3, p17-specific antibody
  • Documents
  • Description
    Caspase 3, p17-specific Rabbit Polyclonal antibody. Positive WB detected in Tunicamycin treated HepG2 cells, HepG2 cells, Tunicamycin treated HeLa cells. Positive FC detected in HeLa cells. Positive IHC detected in human skeletal muscle tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 17 kDa
  • Tested applications
    ELISA, WB, FC, IHC, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    active caspase 3 antibody; Apopain antibody; CASP 3 antibody; CASP3 antibody; Caspase 3 antibody; Caspase 3 subunit p17 antibody; Cleaved Caspase 3 antibody; CPP 32 antibody; CPP32 antibody; CPP-32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; P17 antibody; Protein Yama antibody; SREBP cleavage activity 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_032991). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108875(Caspase 3 Antibody) at dilution of 1:600

    Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108875(Caspase 3 Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:108875(Caspase 3 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:108875(Caspase 3 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:108875(Caspase 3 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:108875(Caspase 3 Antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:108875(Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:108875(Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 HeLa cells were stained with 0.2ug Caspase 3 antibody (Catalog No:108875, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug Caspase 3 antibody (Catalog No:108875, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize p17 of caspase 3 specifically.
  • References
    • Liu M, Xu Y, Han X. Potent effects of flavonoid-rich extract from Rosa laevigata Michx fruit against hydrogen peroxide-induced damage in PC12 cells via attenuation of oxidative stress, inflammation and apoptosis. Molecules (Basel, Switzerland). 19(8):11816-32. 2014.
    • Li J, Liang X, Yang X. Ursolic acid inhibits growth and induces apoptosis in gemcitabine-resistant human pancreatic cancer via the JNK and PI3K/Akt/NF-κB pathways. Oncology reports. 28(2):501-10. 2012.
    • Xiao K, Zou WH, Yang Z. The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats. Cell and tissue research. 359(2):605-18. 2015.
    • Zhao J, Shi Z, Liu S, Li J, Huang W. Ginsenosides Rg1 from Panax ginseng: A Potential Therapy for Acute Liver Failure Patients? Evidence-based complementary and alternative medicine : eCAM. 2014:538059. 2014.
    • Xie X, Zhang J, Chen D. Effects of ghrelin on postresuscitation brain injury in a rat model of cardiac arrest. Shock (Augusta, Ga.). 43(5):490-6. 2015.
    • Chen T, Wang C, Liu Q. Dasatinib reverses the multidrug resistance of breast cancer MCF-7 cells to doxorubicin by downregulating P-gp expression via inhibiting the activation of ERK signaling pathway. Cancer biology & therapy. 16(1):106-14. 2015.
    • Yang L, Shi T, Liu F. REV3L, a promising target in regulating the chemosensitivity of cervical cancer cells. PloS one. 10(3):e0120334. 2015.
    • Wang B, Zhao MZ, Cui NP. Krüppel-like factor 4 induces apoptosis and inhibits tumorigenic progression in SK-BR-3 breast cancer cells. FEBS open bio. 5:147-54. 2015.

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