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Product Name
Caspase 10/p23/17/p12 antibody
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Description
Caspase 10/p23/17/p12 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Apoptosised HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human endometrial cancer tissue. Observed molecular weight by Western-blot: 59 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
ALPS2 antibody; Apoptotic protease Mch 4 antibody; CASP 10 antibody; CASP10 antibody; Caspase 10 antibody; Caspase 10/p23/17/p12 antibody; Caspase10 antibody; FLICE2 antibody; ICE like apoptotic protease 4 antibody; MCH4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Caspase 10/p23/17/p12 recombinant protein (Accession Number: NM_032977). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108871(CASP10 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:108871(CASP10 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:108871(CASP10 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CASP10 antibody Catalog No:108871 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-Caspase 10 (IP:Catalog No:108871, 4ug; Detection:Catalog No:108871 1:300) with HeLa cells lysate 3200ug.
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Background
CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full lengthe has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.
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