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Product Name
CARS antibody
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Description
CARS Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human lung cancer tissue. Positive WB detected in HeLa cells, A549 cells. Observed molecular weight by Western-blot: 70-85 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
CARS antibody; CARS1 antibody; CYSRS antibody; Cysteine tRNA ligase antibody; cysteinyl tRNA synthetase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CARS recombinant protein (Accession Number: NM_001751). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108863(CARS antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:108863(CARS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:108863(CARS antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using CARS antibody Catalog No:108863 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CARS represents cysteinyl-tRNA synthetase
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