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Product Name
Cardiac Troponin T antibody
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Description
Cardiac Troponin T Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, human heart tissue, rat heart tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 34-40 kDa
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Tested applications
ELISA, IF, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cardiac Troponin T antibody; CMH2 antibody; cTnT antibody; TNNT2 antibody; TnTC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cardiac Troponin T recombinant protein (Accession Number: NM_001406726). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human heart using Catalog No:108860(TNNT2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TNNT2 antibody Catalog No:108860 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108860(cTnT Antibody) at dilution of 1:1000
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Background
TNNT2 represents troponin T type 2 (cardiac)
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References
- Li L, Gong H, Yu H. Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes. Journal of cellular biochemistry. 113(12):3788-96. 2012.
- Park JH, Kang HJ, Kang SI. A multifunctional protein, EWS, is essential for early brown fat lineage determination. Developmental cell. 26(4):393-404. 2013.
- Xue Y, Yan Y, Gong H. Insulin-like growth factor binding protein 4 enhances cardiomyocytes induction in murine-induced pluripotent stem cells. Journal of cellular biochemistry. 115(9):1495-504. 2014.
- Kirchhefer U, Brekle C, Eskandar J. Cardiac function is regulated by B56α-mediated targeting of protein phosphatase 2A (PP2A) to contractile relevant substrates. The Journal of biological chemistry. 289(49):33862-73. 2014.
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