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Product Name
CARD11 antibody
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Description
CARD11 Rabbit Polyclonal antibody. Positive IF detected in L02 cells. Positive IHC detected in human lymphoma tissue, human colon cancer tissue. Positive WB detected in L02 cells. Observed molecular weight by Western-blot: 133 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BIMP3 antibody; CARD11 antibody; Carma 1 antibody; CARMA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CARD11 recombinant protein (Accession Number: NM_001324281). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:108854(CARD11 Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:108854(CARD11 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:108854(CARD11 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of L02 cells using Catalog No:108854(CARD11 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.
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