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Product Name
CAP1 antibody
- Documents
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Description
CAP1 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in mouse skin tissue, A431 cells. Positive FC detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 52kd
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Adenylyl cyclase assOCiated protein 1 antibody; CAP antibody; CAP 1 antibody; CAP1 antibody; CAP1 PEN antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CAP1 recombinant protein (Accession Number: NM_001350477). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skin tissue were subjected to SDS PAGE followed by western blot with Catalog No:108932(CAP1 antibody) at dilution of 1:500
IP Result of anti-CAP1 (IP:Catalog No:108932, 3ug; Detection:Catalog No:108932 1:500) with A431 cells lysate 1520ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:108932(CAP1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:108932(CAP1 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug CAP1 antibody (Catalog No:108932, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CAP1 represents CAP, adenylate cyclase-associated protein 1 (yeast)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"