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Product Name
CAMKK2 antibody
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Description
CAMKK2 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive WB detected in PC-3 cells, fetal human brain tissue, HeLa cells, human brain tissue, human heart tissue, Jurkat cells. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CaM kinase kinase 2 antibody; CaM kinase kinase beta antibody; CaM KK 2 antibody; CaM KK beta antibody; CAMKK antibody; CaMKK 2 antibody; CaMKK beta antibody; CAMKK2 antibody; CAMKKB antibody; KIAA0787 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CAMKK2 recombinant protein (Accession Number: NM_172226). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108924(CAMKK2 antibody) at dilution of 1:500
Immunofluorescent analysis of PC-3 cells using Catalog No:108924(CAMKK2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.
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References
- Zhao F, Huang W, Zhang Z. Triptolide induces protective autophagy through activation of the CaMKKβ-AMPK signaling pathway in prostate cancer cells. Oncotarget. 2015.
- Knight JR, Bastide A, Roobol A. Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation. The Biochemical journal. 465(2):227-38. 2015.
- Xi Y, Wu M, Li H. Baicalin Attenuates High Fat Diet-Induced Obesity and Liver Dysfunction: Dose-Response and Potential Role of CaMKKβ/AMPK/ACC Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 35(6):2349-59. 2015.
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