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Product Name
CaMKII alpha-Specific antibody
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Description
CaMKII alpha-Specific Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in SH-SY5Y cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
CaM kinase II subunit alpha antibody; CaMK II subunit alpha antibody; CAMK2A antibody; CAMKA antibody; CaMKII alpha antibody; KIAA0968 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015981). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108915(CAMK2A-Specific antibody) at dilution of 1:1000
IP Result of anti-CAMK2A-Specific (IP:Catalog No:108915, 4ug; Detection:Catalog No:108915 1:1000) with HEK-293 cells lysate 1480ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:108915(CAMK2A-Specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:108915(CAMK2A-Specific Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:108915(CAMK2A-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CaMKII alpha, also named as CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
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