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Product Name
CAMK2 antibody
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Description
CAMK2 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human renal cell carcinoma tissue. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CaM kinase II subunit alpha antibody; CaMK II subunit alpha antibody; CAMK2 antibody; CAMK2A antibody; CAMKA antibody; CaMKII alpha antibody; KIAA0968 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CAMK2 recombinant protein (Accession Number: NM_171825). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108913(CAMK2A antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:108913(CAMK2A Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:108913(CAMK2A Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:108913(CAMK2A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The antibody 13730-1-AP can recognize the members of CAMK2 family.
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