Product Name

Calumenin antibody

Documents

Description

Calumenin Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human placenta tissue, human brain tissue, human lung tissue, human ovary tissue, human skeletal muscle tissue, human spleen tissue. Positive WB detected in HEK-293 cells, HepG2 cells, human placenta tissue, K-562 cells, mouse kidney tissue. Observed molecular weight by Western-blot: 47 kDa, 57 kDa

Tested applications

ELISA, WB, IHC, IF

Species reactivity

Human, Mouse; other species not tested.

Alternative names

CALU antibody; Calumenin antibody; CrOCalbin antibody; FLJ90608 antibody; IEF SSP 9302 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Calumenin recombinant protein (Accession Number: NM_001219). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:50-1:500

Validations

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108911(CALU antibody) at dilution of 1:800

Immunohistochemical of paraffin-embedded human placenta using Catalog No:108911(CALU antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human placenta using Catalog No:108911(CALU antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:108911(CALU Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

CALU represents calumenin

References

Wang Q, Shen B, Chen L. Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis. Oncogene. 34(8):1006-18. 2015.

Calumenin antibody

Product Information

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