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Product Name
Calpain 2 antibody
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Description
Calpain 2 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 80kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Calpain 2 antibody; Calpain 2 catalytic subunit antibody; Calpain 2 large subunit antibody; Calpain large polypeptide L2 antibody; Calpain M type antibody; CANP 2 antibody; CANP2 antibody; CANPL2 antibody; CANPml antibody; CAPN2 antibody; FLJ39928 antibody; M calpain antibody; mCANP antibody; Millimolar calpain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Calpain 2 recombinant protein (Accession Number: NM_001748). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:108835(CAPN2 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108835(CAPN2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody Catalog No:108835 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.
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