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CA12 antibody

Cat.#: 108744

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Product Information

  • Product Name
    CA12 antibody
  • Documents
  • Description
    CA12 Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells, C6 cells, HEK-293 cells, PC-3 cells. Positive FC detected in HEK-293 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 44kd,75-80kd
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CA XII antibody; CA12 antibody; Carbonate dehydratase XII antibody; Carbonic anhydrase 12 antibody; carbonic anhydrase XII antibody; CAXII antibody; FLJ20151 antibody; HsT18816 antibody; Tumor antigen HOM RCC 3.1.3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CA12 recombinant protein (Accession Number: NM_206925). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108744(CA12 antibody) at dilution of 1:300

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108744(CA12 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108744(CA12 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108744(CA12 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108744(CA12 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:108744(CA12 antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 HEK-293 cells were stained with 0.2ug CA12 antibody (Catalog No:108744, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HEK-293 cells were stained with 0.2ug CA12 antibody (Catalog No:108744, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
  • References
    • Asparuhova MB, Secondini C, Rüegg C, Chiquet-Ehrismann R. Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling. Molecular oncology. 9(8):1510-27. 2015.
    • Hong JH, Muhammad E, Zheng C. Essential role of carbonic anhydrase XII in secretory gland fluid and HCO3 (-) secretion revealed by disease causing human mutation. The Journal of physiology. 593(24):5299-312. 2015.
    • Jeong YS, Hong JH. Governing effect of regulatory proteins for Cl(-)/HCO3(-) exchanger 2 activity. Channels (Austin, Tex.). 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"