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Product Name
C9orf86 antibody
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Description
C9orf86 Rabbit Polyclonal antibody. Positive WB detected in MDA-MB-453s cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 125 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
bA216L13.9 antibody; C9orf86 antibody; FLJ10101 antibody; FLJ13045 antibody; Parf antibody; Partner of ARF antibody; pp8875 antibody; RBEL1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of C9orf86 recombinant protein (Accession Number: BC002945). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:108805(C9orf86 Antibody) at dilution of 1:600
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:108805(C9orf86 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]
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