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Product Name
C16orf84 antibody
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Description
C16orf84 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IHC detected in human testis tissue, human kidney tissue. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells. Observed molecular weight by Western-blot: 65-68 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C16orf84 antibody; CTU2 antibody; NCS2 antibody; UPF0432 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of C16orf84 recombinant protein (Accession Number: NM_001012759). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108610(C16orf84 Antibody) at dilution of 1:600
IP Result of anti-C16orf84 (IP:Catalog No:108610, 4ug; Detection:Catalog No:108610 1:500) with HepG2 cells lysate 3600ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108610(C16orf84 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:108610(C16orf84 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:108610(C16orf84 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.
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